Software

TRABI

TRABI is an analysis tool for ImageJ that allows the determination of accurate single-molecule fluorescence intensities independent of the axial position. It analyses single-molecule TIF-stacks (i.e., dSTORM, PALM acquisitions) on the basis of localization files from rapidSTORM, ThunderSTORM, or PeakFit. Besides intensity estimation, which is important for the characterization of novel dyes and switching buffers, it also allows to extract 3D information from 2D images. The software code is open-source and can be downloaded (by clicking on the version number).

Version Changes Release date
1.2 Batch mode bug fix 19 Apr 2017
1.1 Batch processing option, CSV file handling, editable exclusion zone, faster analysis, pixel interpolation in rapidSTORM 11 Apr 2017
1.0 21 Nov 2016

The corresponding paper can be found here. For bug reports, suggestions and improvements please contact Sebastian.

Troubleshooting:

  • TRABI v1.0 only handles TXT files, not CSV files (optional ThunderSTORM output). Simply rename your file test.csv to test.txt to avoid the error:
    beginIndex>endIndex in line 508

 

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